Review of the reproductive biology of amphipods and their endocrine regulation: Identification of mechanistic pathways for reproductive toxicants

Abstract

The reproductive biology of amphipods is reviewed to update the knowledge of the male and female reproductive processes of oogenesis and spermatogenesis as well as the endocrine systems of amphipods with the aim of advancing studies of reproductive toxicology. The ovarian and reproduction cycles of female gammaridean amphipods are closely correlated with the molt cycle, which is under direct control by the steroid hormone 20-hydroxyecdysone. The ability of males to copulate and subsequently for females to ovulate is restricted to the early postmolt period of the females. New developments in our understanding of the molt cycle and the endocrine regulatory pathways for reproduction using genomics techniques on other crustacean species are also discussed. The arthropod sterol ponasterone A or xenobiotics such as the fungicide fenarimol have been shown to elicit endocrine disruption in some crustaceans by acting as an agonist for 20-hydroxyecdysone at the ecdysone receptor or by inhibiting the synthesis of 20-hydroxyecdysone, respectively, resulting in disruption of molting and reproduction. Recent studies suggest that cadmium can inhibit secondary vitellogenesis in amphipods. Experimental approaches for examining the metabolic pathways associated with ecdysteroid hormonal signaling or metabolism, exoskeleton maintenance and molting, and the regulation of vitellogenin in amphipods are discussed. This information should aid in the identification of useful biomarkers for reproductive toxicity.