Abstract
The absorption changes of bacteriochlorophyll in the chromatophores of Chromatium D were investigated. Addition of non-ionic and cationic detergents and alcohols to the chromatophore suspension caused a conversion of B850 to B810. A similar result was obtained with treatments which reduce the water content of the chromatophores ( e.g., drying, treatment with higher concentrations of neutral salts or glycerol). On reducing the concentration of the detergents and alcohols in the chromatophore suspension by dialysis, the absorption spectrum of the chromatophores completely returned to its original state. A similar recovery of absorption spectrum was obtained by wetting the dried films of chromatophores or by diluting the salts or glycerol solution with water. No significant changes occurred in B890 during these interconversions. These treatments also induced reversible changes in the fluorescence emission spectrum of bacteriochlorophyll and the action spectrum of photo-induced oxidation of c-type cytochrome in the chromatophore.
Published Version
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