Abstract
Summary Protoplasmic streaming within barley (Hordeum vulgare L.) and tomato (Lycopersicum esculentum Mill.) root hairs was investigated after incubation with increasing concentrations of the inhibitor of contractile protein filaments, cytochalasin B (CB). A lower concentration of CB (1.0,βg/ml) had no inhibitory effect on protoplasmic streaming, whereas 5.0 to 10.0 μg/ml caused a progressive inhibition up to stopping of the streaming. At the highest CB-concentration used, side effects of CB became apparent like swelling, aggregation and vacuolisation of the cytoplasm. After removal of CB by washing with phosphate buffer, a complete recovery of streaming and disappearence of the morphological effects were observed. These results support the assumption that contractile protein filaments are in- volved in the protoplasmic streaming of root hairs.
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