Reversible deafferentation of the zebrafish olfactory bulb with wax plug insertion

Abstract

BackgroundDeafferentation of the zebrafish olfactory bulb allows investigation of neuroplasticity in a particularly dynamic brain region of a popular model animal known for its regenerative abilities. Current methods to remove sensory input to the zebrafish olfactory bulb differ in the extent of deafferentation and potential for recovery. New methodWe present a novel method of olfactory bulb deafferentation using continuous wax plug insertions into the nasal cavity of zebrafish. Wax plugs were placed in the nasal cavity and replaced if needed over 1wk or 3wk survival periods. Wax plugs were removed from fish after 1wk of occlusion to analyze the potential recovery of the olfactory organ and bulb. ResultsWax plug insertions caused a dramatic reduction in olfactory organ size and structure and significantly reduced afferent input to the olfactory bulb after 1wk and 3wk. Removal of the wax plugs after 1wk allowed for recovery of the olfactory organ and subsequent reinnervation of the olfactory bulb. Comparisons with existing methodsChemical ablation with detergent causes partial, temporary deafferentation of the olfactory bulb. Cautery ablation causes complete, permanent deafferentation of the olfactory bulb. Wax plug insertions cause nearly complete, temporary deafferentation, allowing both significant deafferentation and the potential for reinnervation of the olfactory bulb. ConclusionsThe wax plug insertion method of deafferentation described here is unique in that it destroys almost completely the structure of the olfactory organ and removes almost completely sensory input to the olfactory bulb, yet the organ returns to its typical morphology and afferent innervation returns.