Abstract
To find optimal biospecific hydroxyalkyl methacrylate (Spheron) derivatives for reversible and irreversible immobilization of car☐ypeptidase Y, we elaborated the procedure for the biospecific sorption of car☐ypeptidase Y to Spheron—Gly-Gly- p-aminobenzylsuccinic acid, mercury—Spheron and concanavalin A—Spheron. The highest yield was achieved in the case of concanavalin A—Spheron, which was also used for the irreversible, oriented immobilization by cross-linking of car☐ypeptidase Y and concanavalin A with glutaraldehyde.
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