Abstract
Since mercuric mercury (Hg 2+) and methylmercury (CH 3Hg +) display different toxicological properties in mammals, methods for their quantification in dietary items must be available. Employing Hg-specific detection, we have developed a rapid, isocratic, and affordable RP-HPLC separation of these mercurials using thiol-containing mobile phases. Optimal separation was achieved with a 50 mM phosphate-buffer containing 10 mM l-cysteine at pH 7.5. The separation is driven by the on-column formation of complexes between each mercurial and l-cysteine, which are then separated according to their different hydrophobicities. The developed method is compatible with inductively coupled plasma atomic emission spectrometry and was applied to analyze spiked human urine.
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