Abstract
Under standardized conditions, the combined montoring of UV absorbance ( A 280) and fluorescence intensity ( I F) at 390 or 393 nm (on excitation at 300 or 340 nm, respectively) provides a diagnostic criterion for the identification of cinnamic and benzoic acid derivatives by reversed-phase high-performance liquid chromatographic separations of natural phenolic acids. The method can easily be used to determine the concentrations of these compounds if 3,5-dinitrobenzoic acid is applied as an internal standard. When applied to a saponified rye kernel water extract, ferulic, p-coumaric, sinapic, syringic and vanillic acids were shown to be present.
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