Revealing the effects of fermented food waste on the growth and intestinal microorganisms of black soldier fly (Hermetia illucens) larvae

Abstract

The escalating global food waste (FW) issues necessitate sustainable management strategies. Black soldier fly larvae (BSFL) offer a promising solution for FW management by converting organic matter into insect protein. However, the fermentation of FW during production, collection, and transportation induces changes in FW’s physicochemical properties and bacterial communities, requiring further exploration of its impact on BSFL growth and gut microbiota. The results showed that feeding FW fermented for different durations (0–10 d) slightly affected the BSFL yield. Feeding FW fermented for 8 d, characterized by a lower pH and higher biodiversity, resulted in a slight increase in larval biomass (222 mg/larvae). Nearly all groups harvested the peak larval biomass after 10 day’s bioconversion. The fermentation significantly altered the microbial community of FW, with an increase in the abundance of unclassified_f_Clostridiaceae and a decrease in Lactobacillus abundance. As bioconversion progressed, intricate and mutualistic microbial interactions likely occurred between the BSFL gut and FW substrate, restructuring each other’s microbial community. Specifically, the abundance of unclassified_f_Clostridiaceae increased in the BSFL gut, while its abundance in the initial larval gut was extremely low (<1 %). Despite the substrate microbial changes and interactions, a stable core gut microbiota was identified across all BSFL samples, primarily composed of nine genera dominated by Enterococcus and Klebsiella. This core gut microbiome may play a crucial role in facilitating the adaptation of BSFL to various environmental conditions and maintaining efficient FW bioconversion. These findings enhance our understanding of the role of BSFL gut microbiota in FW bioconversion.