Revealing the deep complexity of the human transcriptome with targeted sequencing

Abstract

Event Abstract Back to Event Revealing the deep complexity of the human transcriptome with targeted sequencing Timothy Mercer1*, I Deveson1 and M Brunck1 1 Garvan Institute of Medical Research, Genomics and Epigenetics Division, Australia The human genome encodes an unknown diversity of protein-coding and noncoding RNAs. This diversity and the wide range of gene expression limit the capacity of RNA sequencing to resolve the transcriptome and, as a result, current reference catalogs provide an incomplete profile of human gene content. Here, we target RNA sequencing to human chromosome 21 and syntenic regions of the mouse genome to provide a complete and unbiased cross-section that indicates the size, structure and composition of the mammalian transcriptome. We find that the majority of transcripts are noncoding RNAs that are not represented in current catalogs. These noncoding RNA populations have diverged between human and mouse, and orthologs are rare. Noncoding exons, in contrast to coding exons, are near-universally alternatively spliced to generate an inexhaustible diversity of isoforms. This noncoding isoform diversity is regulated by local cis-elements that are sufficient to recapitulate human alternative splicing profiles in a mouse cell. Our results indicate the full breadth, diversity and plasticity of information encoded in the mammalian genome and reveal distinctions in the organization, splicing and evolution of its coding and noncoding content. Keywords: Gene Expression, RNA, noncoding RNA, human transcriptome, targeted sequencing Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: Symposium 4: The Living Chemistry of RNA Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Mercer T, Deveson I and Brunck M (2016). Revealing the deep complexity of the human transcriptome with targeted sequencing. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00019 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 26 Jul 2016; Published Online: 11 Aug 2016. * Correspondence: Dr. Timothy Mercer, Garvan Institute of Medical Research, Genomics and Epigenetics Division, Darlinghurst, Sydney, Australia, t.mercer@garvan.org.au Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Timothy Mercer I Deveson M Brunck Google Timothy Mercer I Deveson M Brunck Google Scholar Timothy Mercer I Deveson M Brunck PubMed Timothy Mercer I Deveson M Brunck Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.