MiRNA-331-5p expression level is altered in neuronal cell under elevated oxidative stress

Abstract

Event Abstract Back to Event MiRNA-331-5p expression level is altered in neuronal cell under elevated oxidative stress Pei Ling Yeo1, Soi Moi Chye2, Rhun Yian Koh2, Kenny G. Voon3 and Khuen Yen Ng4, 5* 1 International Medical University, Postgraduate Studies and Research, Malaysia 2 International Medical University, Department of Human Biology, Malaysia 3 International Medical University, Department of Pathology, Malaysia 4 Monash University Malaysia, Jeffrey Cheah School of Medicine and Health Sciences, Malaysia 5 Monash University Malaysia, School of Pharmacy, Malaysia Parkinson’s Diseases (PD) is a debilitating neurodegenerative disorder causing movement and neurological disabilities. Apoptotic death of the dopaminergic neurons in the substantia nigra pars compacta region of midbrain of PD patients are known to be the devastating events following the buildup of oxidative stress. MicroRNA (miRNA) is a class of small, non-coding RNA which plays important roles in regulating gene expression post-transcriptionally. Increasing evidence suggested that miRNA profile might be altered in PD. A recent investigation reported that miRNA-331-5p was significantly elevated (~ 22 folds of that in controls) in the blood plasma of PD patients. However, no study has been conducted to understand the pathological involvement of this miRNA in PD. Thus, this study aims to investigate whether levels of miRNA-331-5p in neuronal cells are affected under high oxidative stress. In this study, neurotoxin, 6-hydroxydopamine (6-OHDA) was added to SH-SY5Y cells for 6, 12, and 24 hours to induce oxidative stress. Cell viability was assessed with MTT assay whereas levels of reactive oxygen species (ROS) was quantified with DCFH-DA fluorescent probe staining. Expression of miRNA-331-5p in the cells was quantified using quantitative Real-time PCR. Result shows that 6-OHDA treatment caused a time-dependent increased in ROS generation most pronounced in 100 and 200 µM (up to 6 folds) treatment groups. 6-OHDA treatment also resulted in a time-dependent decreased of cell viability to 7% across all concentration tested. Subsequently, miRNA expression level showed significant up-regulation with 12 and 24 hours treatment groups. This increase in miRNA-331-5p level is correlated to the increase in ROS level and neuronal cell death. In conclusion, these findings suggest that miRNA-331-5p might be involved in the pathogenesis of PD as its expression is altered under elevated oxidative stress. Keywords: Oxidative Stress, miRNA, Pathogenesis, Parkinson’s disease, neurodegeneration Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: Poster Presentation Session Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Yeo P, Chye S, Koh R, Voon KG and Ng K (2016). MiRNA-331-5p expression level is altered in neuronal cell under elevated oxidative stress. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00119 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Aug 2016; Published Online: 11 Aug 2016. * Correspondence: Dr. Khuen Yen Ng, Monash University Malaysia, Jeffrey Cheah School of Medicine and Health Sciences, Bandar Sunway, Selangor, Malaysia, ng.khuen.yen@monash.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Pei Ling Yeo Soi Moi Chye Rhun Yian Koh Kenny G Voon Khuen Yen Ng Google Pei Ling Yeo Soi Moi Chye Rhun Yian Koh Kenny G Voon Khuen Yen Ng Google Scholar Pei Ling Yeo Soi Moi Chye Rhun Yian Koh Kenny G Voon Khuen Yen Ng PubMed Pei Ling Yeo Soi Moi Chye Rhun Yian Koh Kenny G Voon Khuen Yen Ng Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.