Revealed interactive association between macro-molecular structures and true nutrition supply in cool-season adapted CDC chickpeas and CDC barley using advanced vibrational molecular spectroscopic techniques

Abstract

Recently, an interactive association between molecular structures and nutrition of protein could be used for evaluation of truly absorbable nutrient supply, nutrient utilization and availability. However, there was no study found in cool-season adapted chickpeas. The objectives of this study were to: (1) reveal an interactive association between macro-molecular structures and nutrition of protein in cool-season adapted CDC chickpeas; (2) Characterize macro-molecular structure features of cool-season adapted CDC chickpeas and their genotypic impact on nutrient profile, fractionation, bioenergy values and biodegradation function in comparison with CDC barley kernel. Macromolecular structures determination was carried out using molecular vibrational spectroscopy-ATR-FTIR. Results showed that with vibrational molecular spectroscopy, the macromolecular spectral characteristics could be revealed among the varieties and between types of CDC developed grain (Chickpea vs CDC barley). The molecular spectral characteristics had significant relationship (P < 0.05) with carbohydrate profiles, sub-fractions and degradation, bypassed, total digestible carbohydrate and protein. Multiple regression with model nutrient variable selection study showed that protein and carbohydrate-related macro-molecular structural parameters were associated with nutrient profiles and degradation characteristics. The results indicated that there was an interactive association between macro-molecular structures and nutrition of protein in cool-season chickpeas.