Abstract
Protoplasm from Bryopsis maxima, a coenocytic green alga, was dissociated into two fractions; chloroplasts and a protoplasmic fraction without chloroplasts (PF). PF induced nuclei, mitochondria, dictyosome, endplasmic reticulum, etc. These two fractions were reunited and protoplasts with complete plasmamembrane were reformed within 10 h. Cell wall regeneration was observed 24–30 h after reuniting. New cells began budding 2–3 days after, and 1 month after reunification, they had developed into mature plants.
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