Abstract
Canine parvovirus 2 (CPV-2) outbreaks in close quarters such as kennels or shelters can cause substantial case fatality. Thirteen dead Labradors from a secluded kennel of security dogs presented with typical clinical signs and gross pathology of parvovirus infection. Whole genome shotgun sequencing from tissue-extracted genomic DNA detected new CPV-2a as the contributing antigenic variant. Further genotyping using polymerase chain reaction coupled with high-resolution melt assays (PCR-HRM) confirmed new CPV-2a infection in all deceased dogs. PCR-HRM of additional thirty-four clinically suspected dogs suggested that this variant is in wider community circulation, at least in the southeastern part of Bangladesh. We present complete genome sequence of the new CPV-2a variant circulating in the domestic canine population of Bangladesh.
Highlights
Canine parvovirus 2 (CPV-2) is one of the deadly viral pathogens that cause severe hemorrhagic gastroenteritis and lymphopenia in domestic dogs, in young, unvaccinated puppies
CPV is a non-enveloped, single-stranded DNA virus that emerged in the late 1970s from a feline parvovirus (FPV)-like ancestral virus, probably via an interspecies jump to domesticated dogs [1,3]
Whole genome shotgun (WGS) sequencing of the pooled Genomic DNA (gDNA) and subsequent metagenomic analysis unveiled that approximately 58% of non-host-derived reads were mapped to viruses from species Carnivore protoparvovirus 1, 14% from enteric bacteria under family Enterobacteriaceae, and the rest from Eukaryotic biota, likely ingesta
Summary
Canine parvovirus 2 (CPV-2) is one of the deadly viral pathogens that cause severe hemorrhagic gastroenteritis and lymphopenia in domestic dogs, in young, unvaccinated puppies. According to the most recent classification, CPV is included in the family Parvoviridae, subfamily Parvovirinae, genus Protoparvovirus, which forms a unique species, Carnivore protoparvovirus 1, along with feline parvovirus and other parvoviruses of carnivores [2]. CPV is a non-enveloped, single-stranded DNA virus that emerged in the late 1970s from a feline parvovirus (FPV)-like ancestral virus, probably via an interspecies jump to domesticated dogs [1,3]. The linear single-stranded DNA (ssDNA) genome of CPV-2 is about 5 kb in length, possess two open reading frames (ORFs) that encodes for two non-structural proteins (NS1 and NS2), and two structural proteins (VP1 and VP2) which forms the viral capsid [4,5]
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