Abstract
The aim of this study is to examine whether molecular hydrogen (H2) is able to reduce oxidative stress after corneal damage induced by UVB irradiation. We previously found that UVB irradiation of the cornea caused the imbalance between the antioxidant and prooxidant enzymes in the corneal epithelium, followed by the imbalance between metalloproteinases and their physiological inhibitors (imbalances in favour of prooxidants and metalloproteinases) contributing to oxidative stress and development of the intracorneal inflammation. Here we investigate the effect of H2 dissolved in PBS in the concentration 0.5 ppm wt/vol, applied on rabbit corneas during UVB irradiation and healing (UVB doses 1.01 J/cm2 once daily for four days). Some irradiated corneas remained untreated or buffer treated. In these corneas the oxidative stress appeared, followed by the excessive inflammation. Malondiladehyde and peroxynitrite expressions were present. The corneas healed with scar formation and neovascularization. In contrast, in H2 treated irradiated corneas oxidative stress was suppressed and malondiladehyde and peroxynitrite expressions were absent. The corneas healed with the restoration of transparency. The study provides the first evidence of the role of H2 in prevention of oxidative and nitrosative stress in UVB irradiated corneas, which may represent a novel prophylactic approach to corneal photodamage.
Highlights
T The aim of this study is to examine whether molecular hydrogen (H2) is able to reduce oxidative stress after corneal damage induced by UVB irradiation
Apart from the group of rabbits with UVB irradiated corneas treated with H2 solution or with PBS (H2 free), there was the group of rabbits, which were left without any treatment during and after UVB irradiation
Biochemical and macroscopical results of irradiated untreated corneas did not significantly differ from the results obtained with irradiated corneas treated with PBS (H2 free), we did not show the results of the irradiated untreated group
Summary
Thopxhyhidoedrrtaoaotpgdiveeaeunmtsiiantcrgceeeosffsrenacentadol fUcmoVroBnle-eiIanclCudluacLredE CestmirCejka1,JanKossl1,2,BarboraHermankova1,2,VladimirHolan1,2,SarkaKubinova[1], John H. Some irradiated corneas remained untreated or buffer treated In these corneas the oxidative stress appeared, followed by the excessive inflammation. After UVB irradiation, corneal epithelial ROS-generating oxidases contribute to the antioxidant/prooxidant imbalance, in favour of prooxidants, and to the oxidative stress in the cornea[2,3,4,5,6,7]. E untreated or PBS treated UVB irradiated corneas, whereas after H2 treatment beneficial results in corneal healing were obtained. In this study H2 prevented or highly reduced the oxidative damage of UVB irradiated corneas, leading to the restoration of transparency. The corneas healed without neovascularization and scar formation This was in contrast to irradiated untreated or PBS treated corneas, which were untransparent and vascularized
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