Abstract
BackgroundGastric cancer receives considerable attention not only because it is the most common cancer all through the world, but also because it’s on the top third leading reason for cancer-related death. Lidocaine is a well-documented local anesthetic that has been reported to suppress cancer development. The study explored the effects of lidocaine on the growth, migration and invasion of the gastric carcinoma cell line MKN45 and the mechanism behind.MethodsThe effect of lidocaine on viability, proliferation and apoptosis of MKN45 cells were analyzed by Cell Counting Kit-8 assay, BrdU staining assay and flow cytometry, respectively. Moreover, cell migration and invasion were both examined by Transwell assay. The expression of apoptosis-, migration-, and invasion-related proteins were detected by western blot. The relative expression of miR-145 was determined by qRT-PCR. Moreover, the impact which lidocaine brought on MEK/ERK and NF-κB pathways were examined by western blot.ResultsLidocaine inhibited viability, proliferation, migration, and invasion of MKN45 cells, while enhanced apoptosis. Moreover, miR-145 expression was enhanced by lidocaine; and transfection with miR-145 inhibitor increased cell viability, proliferation, migration, and invasion, but inhibited apoptosis. The up-regulation of miR-145 was partly contributed to the inhibitory effect of lidocaine on gastric cancer cell line MKN45. Finally, lidocaine inactivated MEK/ERK and NF-κB pathways via up-regulation of miR-145.ConclusionsOur results suggested that lidocaine decreased growth, migration and invasion of MKN45 cells via regulating miR-145 expression and further inactivation of MEK/ERK and NF-κB signaling pathways.
Highlights
Gastric cancer receives considerable attention because it is the most common cancer all through the world, and because it’s on the top third leading reason for cancer-related death
Lidocaine inhibited growth of MKN45 cells The MKN45 cell viability, proliferation, and apoptosis were determined after cells were treated by lidocaine
According to Cell counting Kit-8 (CCK-8) assay, cell viability was inhibited after cells were cultured with different concentrations of lidocaine (1, 5 and 10 mM) (Fig. 1a)
Summary
Gastric cancer receives considerable attention because it is the most common cancer all through the world, and because it’s on the top third leading reason for cancer-related death. Lidocaine is a well-documented local anesthetic that has been reported to suppress cancer development. The study explored the effects of lidocaine on the growth, migration and invasion of the gastric carcinoma cell line MKN45 and the mechanism behind. Even though the incidence rate in the past years is declined, gastric carcinoma is still standing for over 1,000,000 new cases in 2018 and an approximately 783,000 deaths, which made it to be one of the most common diagnosed cancer with high mortality [1]. Lidocaine has been showed to inhibit growth and metastasis in various cancers. Lidocaine could inhibit proliferation of bladder cancer BIU-87 cell line in a dose-dependent manner and enhance the actions of
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