RETRACTED ARTICLE: Karyotype analysis and identification of extra chromosomes in primary aneuploid stocks of grass pea (Lathyrus sativus L.) by fluorescence chromosome banding

Abstract

AbstractProminent primary aneuploid stocks namely seven primary trisomics, seven primary tetrasomics, and six double trisomic types were earlier developed in grass pea (Lathyrus sativus L.), a hardy legume. Despite distinct morphological features, identity and nature of their extra chromosome(s) were elusive, hampering assignment of desirable breeding traits into specific linkage groups. The present study aims to analyze the banding pattern and to reveal the identity of extra chromosome(s) involved in these 20 aneuploid types in grass pea. Conventional orcein banding was first done using the root-tip squash technique in all aneuploids along with disomic (2n = 2x = 14) parent, and karyomorphological features were noted. Chromosomes were classified following the total length of individual chromosomes and arranged in order of decreasing sizes, keeping their centromeres in a straight line. DNA-base specific chromomycin A3 (CMA) and 4,6ʹdiamidino-2-phenylindole (DAPI) banding pattern were finally employed to c...