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Abstract
BackgroundMicroRNAs (miRNAs) play key roles in cancer development and progression. The purpose of the present study was to determine the expression levels of miR-133a and miR-539 in osteosarcoma patients and to further investigate the clinicopathological, and prognostic value of these miRNAs.MethodsThe expression levels of miR-133a and miR-539 were determined by qRT-PCR. Associations between miRNAs expressions and various clinicopathological characteristics were analyzed. Survival rate was determined with Kaplan–Meier and statistically analyzed with the log-rank method between groups. Survival data were evaluated through multivariate Cox regression analysisResultsOur findings revealed that the miR-133a expression was significantly decreased in clinical osteosarcoma tissues compared to adjacent normal bone tissues. The expression level of miR-539 was decreased in clinical osteosarcoma tissues as compared to those adjacent normal tissues. Low expressions of miR-133a and miR-539 were significantly association with advanced TNM stage (P = 0.002; P = 0.001), and metastasis or recurrence (P = 0.001; P = 0.01). Furthermore, Kaplan–Meier survival analysis and log-rank test showed that the low expressions of miR-133a and miR-539 were correlated with the reduced overall survival of osteosarcoma patients. Multivariate Cox proportional hazards model showed that decreased expressions of miR-133a and miR-539 (P = 0.007; P = 0.02), TNM stage (P = 0.001; P = 0.002), and metastasis or recurrence (P = 0.005; P = 0.026) were independent prognostic markers of overall survival of patients.ConclusionThese results suggest that decreased miR-133a and miR-539 expressions may participate in the progression of osteosarcoma. Together, these results showed that miR-133a and miR-539 may have their role in both progression and prognosis of osteosarcoma.
Highlights
MicroRNAs play key roles in cancer development and progression
As demonstrated by quantitative real-time PCR, the miR-133a expression was significantly decreased in clinical osteosarcoma tissues compared to adjacent normal bone tissues
The expression level of miR-539 was decreased in clinical osteosarcoma tissues as compared to that adjacent normal tissues
Summary
MicroRNAs (miRNAs) play key roles in cancer development and progression. It has been reported that miR-539 may inhibit cell proliferation through suppressing the MITF expression [9]. MiR-133a is shown to be an important regulator in osteogenesis, and its down-regulation has been reported in bone morphogenetic protein (BMP)-induced osteogenesis. It can function as suppressor of RunX2 expression for inhibition of osteoblast differentiation [12]. Further investigations are required to identify miR-133a and miR-539 expression levels in clinical osteosarcoma patients and its potential roles in osteosarcoma carcinogenesis and progression. We examined the clinical importance of miR-133a and miR-539 expressions in osteosarcoma tissue samples using real-time PCR
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