RETRACTED ARTICLE: Upregulation of miR-300 and downregulation of miR-125b act as potential predictor biomarkers in progression, metastasis, and poor prognosis of osteosarcoma.

Abstract

Abstract MiRNAs play crucial roles in development of cancer. However, the underlying mechanisms of miRNAs in osteosarcoma (OS) are poorly understood. This study is aimed to investigate the clinical importance of miR-125b and miR-300 expression in OS tissue samples using real-time PCR.Total RNA was purified from samples and noncancerous bone tissues and then real-time PCR was used to evaluate the expression rate of microRNAs. Furthermore, the association of miR-125b and MiR-300 level with pathological factors and prognosis were evaluated. Our results suggested that miR-125b expression was significantly downregulated in osteosarcoma bone tissue compared with noncancerous bone tissues. On the other hand, miR-300 expression was significantly upregulated in OS bone tissue than noncancerous bone tissues.Our findings suggested that a lower expression level of miR-125b was linked to advanced clinical stage (P = 0.005), metastasis (P = 0.004), and a large size of tumor (P = 0.023). In addition, a higher expression level of miR-300 was related to advanced tumor, node, and metastasis (TNM) stage (P = 0.002), metastasis (P = 0.001), and a large size of tumor (P = 0.038). The lower expression and higher expression of mentioned microRNAs was significantly linked to shorter overall survival according to the Kaplan-Meier survival and log-rank analysis (log-rank test P = 0.026; P = 0.017).Moreover, lower expression of miR-125b (P = 0.001), a decreased expression level of miR-300 (P = 0.004), TNM stage (P = 0. 0.004; P = 0.003), metastasis (P = 0.027; P = 0.001), and a large tumor size (P = 0.006; P = 0.025) were independent prognostic markers of overall survival of patients according to the multivariate Cox proportional hazards model. In conclusion, our results indicated that downregulation of miR-125b and miR-300 was associated with progression of OS and both of them may act as tumor suppressor in OS.