RETRACTED: Биологические свойства грамнегативных бактерий, верифицированных в моче пациенток с рецидивирующей неосложненной инфекцией нижних мочевых путей

Abstract

Article retracted. Uncomplicated infections of the lower urinary tract are more often recorded in women and are characterized by recurrent severe course. The lack of effective approaches to the management of this cohort of patients and the insufficient knowledge of the qualitative characteristics of uropathogens hinder the successful treatment of these infections. The purpose of this project is to study the biological properties of representatives of Gram-negative bacteria isolated from the urine of patients with recurrent uncomplicated lower urinary tract infection at various levels of bacteriuria. We present here the results of the one-stage cohort study of 62 patients 20-50 years old with recurrent uncomplicated lower urinary tract infection. The level of bacteriuria, genes of virulence factors, hemolytic, adhesive and anti-lysozyme activities were determined for the Gram-negative bacteria isolated from the urine of patients. Representatives of Gram-negative microorganisms (62 strains) were isolated from the urine of all patients. Their bacteriuria levels ranged from 10 2 to 10 8 CFU/ml. A wide range of the virulence factor genes with the prevalence of papGII , feoB, fyuA , were found in all strains regardless of the degree of bacteriuria. Twenty significant (p<0.05) coefficients of mutual contingency between different pairs of genes were found. We found that the hemolytic activity of Enterobacteriaceae is statistically significantly associated with papA , feoB , fyuA , kpsMII , and usp , while the anti-lysozyme activity is associated with papA , afa , and usp . The detection of genotypic and some phenotypic traits expressed in various variations in all Gram-negative bacteria regardless of their bacteriuria levels creates a potential risk for recurrent of the lower urinary tract infection. Consequently, even the minimal levels of bacteriuria become clinically significant. Therefore, in bacteriological diagnostics it is necessary to determine all levels of bacteriuria, including the minimum level (10 2 CFU/ml), since isolated microorganisms at this level have not only adhesive and anti-lysozyme activities, but also contain genes encoding the virulence factors.