Abstract
The chromatographic properties of rosmarinic and valerenic acids have been studied on an amino stationary phase with eight binary mobile phases (methanol-water, ethanol-water, propanol-water, acetonitrile-water, acetonitrile-methanol, THF-water, acetonitrile-buffer, and methanol-buffer) in which the concentration of organic modifier was varied from 0 to 100% ( v/v ). The retention of the compounds and the impact on their chromatographic behavior of the solvents used as mobile phases are discussed. The dependence of the R F of valerenic and rosmarinic acids on buffer solution pH was also investigated.
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