Abstract
SUMMARY—The ability of 19 restrictases to produce Re-bands detectable by Giemsa stain in plant chromosomes was tested with Vicia faba, Hordeum vulgare, and Allium cepa. For comparison, some of the restrictases were also used to digest human chromosomes. Additionally, DNasel treatments were performed for uniform and nearly complete digestion of nuclear DNA. DNA extraction by some endonucleases from 14C-labelled nuclei was tested measuring radioactivity of enzyme solutions after treatment of chromosomes. Especially effective in Re-banding of Vicia faba chromosomes were sequential digestions with Alul → Mbol and DdeI → ClaI. Some restrictases did not produce any bands. However, occasionally control treatments with incubation buffers also resulted in dark Giemsa bands after long treatment times. The applicability of Re-banding for plant chromosomes is dicussed.
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