Restricted electron flux increases coenzyme Q 10 production in Agrobacterium tumefaciens ATCC4452

Abstract

Coenzyme Q 10 (CoQ 10) is an electron carrier in the respiration chain with antioxidant activity. The effects of aeration and agitation rates, dissolved oxygen levels, an electron flux inhibitor (azide), a proton gradient releaser (2,4-dinitrophenol (DNP)), and an oxidative stressor (hydrogen peroxide (H 2O 2)) on the intracellular CoQ 10 contents in Agrobacterium tumefaciens ATCC4452 were investigated. With a decrease of dissolved oxygen level from 20 to 5%, the intracellular CoQ 10 content increased about four-fold, yielding 2 mg/g dry cell weight at 5% dissolved oxygen level. Azide significantly increased the intracellular CoQ 10 content, with the highest value of 5.3 mg/g dry cell weight in the presence of 0.45 mM of sodium azide. However, DNP (up to 200 μM) and H 2O 2 (up to 10 μM) did not affect the intracellular CoQ 10 content, indicating proton gradient release, and oxidative stress do not affect the synthesis of CoQ 10. These results show that restricted electron flux by limited oxygen supply and the addition of azide increases the intracellular CoQ 10 content, suggesting a feedback regulation of CoQ 10 biosynthesis by its physiological function of electron carrier in the respiration chain.