Abstract
Leaf formation has been induced in long-term callus cultures no longer able to differentiate under standard conditions by altering the sequence of transfers to culture media which differ in their hormonal composition or glucose concentration. Transfer onto a medium without auxin or hormones 14 days prior to the transfer to the regeneration medium enhances the morphogenetic response in callus cultures maintained for longer than 6 months. An increase of glucose concentration from 2 to 6 % stimulated leaf formation in 6–15 month old tissue culture. Methods are also described which allow the regeneration of whole, phenotypically normal plants from roots or abnormal leaves obtained in long-term callus cultures.
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