Restoration of laboratory animal’s normal microflora with induced dysbiosis

Abstract

The possibility of using autosymbiotic aerococci and their complex as a bacterial basis for probiotic preparations has been studied. Aerococci — A.viridans were selected as probiotic microorganisms being related to a normal microflora. Aerococci have unique properties that distinguish them from a number of common probiotic microorganisms. The aim of the study was to study the effect of autosymbionts A.viridans and their complex on the quantitative and qualitative composition of the intestinal microbiocenosis in experimental dysbiosis induced by long-term administration of ampixus in comparison with A.viridans 167 museums. An experimental model of ampiox-dependent dysbiosis in mice was used to evaluate their effectiveness. have shown Studies changes in the composition of the mice’s microflora after the exposure of ampiox. There was a decrease in the total number of colibacteria, aerococci, bifidobacteria, lactobacilli and an increase of OPM: fungi Candida, Proteus spp., Staphylococcus epidermidis, Enterococcus faecium and Clostridium spp. A comparative study of the dysbiosis correction effectiveness with freshly isolated mice’s autosymbionts (individual strains and a complex preparation containing all clones of the studied autosymbionts) and the heterologous strain A.viridans 167 was carried out. Aerococci were ascertained to restore the microflora of mice with ampiox-dependent dysbiosis to the initial state after 14-day application. Homologous autosymbiotic aerococci, including their complex, and the heterologous strain A.viridans 167 were revealed to effect differently on the ratio of certain components of the microbiocenosis inducing reliable increase of bifidobacteria and lactobacilli content and a decrease of OPM’s one. Autosymbiotic mice’s aerococci, separately and in the complex, showed greater affinity for the mice’s epitheliocytes then heterologous strain №167.