Responses of the root morphology and photosynthetic pigments of ryegrass to fertilizer application under combined petroleum-heavy metal stress.

Abstract

With developments in industry, petroleum and heavy metal pollution are increasingly affecting soil, significantly harming the environment, biosecurity, and human health. Therefore, the remediation of contaminated soil is becoming increasingly important. In this study, ryegrass (Lolium perenne L.) was planted in petroleum-heavy metal co-contaminated soil with the application of nitrogen and phosphorus fertilizers. Three treatments were set up: uncontaminated soil + ryegrass (SH); petroleum-heavy metal co-contaminated soil + ryegrass (SPGH); and petroleum-heavy metal co-contaminated soil + ryegrass + nitrogen and phosphorus fertilizer (SPGH + NP). The results showed that the petroleum-heavy metal co-contamination promoted increases in the root length, surface area, volume, and diameter of ryegrass roots, increasing the below-ground biomass and decreasing the photosynthetic pigment content in the early stages of the experiment. The ratios of chlorophyll a/b and chlorophyll/carotenoid also increased. However, the application of fertilizer reduced the length, surface area, volume, and diameter of ryegrass roots in the co-contaminated soil, and the below-ground biomass decreased while the above-ground biomass increased. Furthermore, the photosynthetic pigment content was significantly higher than that in the unfertilized treatment and the chlorophyll a/b ratio decreased while the chlorophyll/carotenoid ratio increased. Therefore, fertilizers could alleviate the toxic effects of petroleum-heavy metal combined pollution on ryegrass roots and promote the synthesis of chlorophyll and other pigments, thus reducing the inhibitory effect of petroleum-heavy metal combined pollution on ryegrass growth and facilitating the remediation of the polluted soil.