Response to: On methods of determination of homogeneous nucleation rates of protein crystals

Abstract

In their letter, Galkin and Vekilov suggest that our analysis [Dixit et al., Colloids Surf. A 190, 47 (2001)] of their experimental technique [Galkin and Vekilov, J. Phys. Chem. B 103, 10965 (1999); J. Am. Chem. Soc. 122, 156 (2000)] for estimating protein crystal nucleation rates contains inconsistencies. Here, we examine their criticisms and find that our analysis does not contain inconsistencies. We conclude by restating our claim in [Dixit et al., Colloids Surf. A 190, 47 (2001)] that Galkin and Vekilov underestimate protein crystal nucleation rates by several orders of magnitude because of the way their experimental data is interpreted.