Abstract
This study was designed to optimize maceration (ME) and ultrasound-assisted extraction (UAE) parameters (extraction time X1, temperature X2, solvent/mass Ratio X3 and Ethanol concentration X4) of antioxidant activity of Eucalyptus marginata L. leaves’ using response surface methodology (RSM). The antioxidant activity was evaluated using the DPPH assay and ferric reducing antioxidant power (FRAP). The optimal conditions for ME and UAE of antioxidant compounds were: X1 (min) = 88 and 50, X2 (°C) = ~75 and ~79, X3 (ml/g) = 40 and 39.5 and X4 (% of ethanol) = 59.65 and 58.48, respectively. Consequently, these optimized ME and UAE methods have shown a potential application for the efficient extraction of polyphenolic antioxidants from Eucalyptus marginata L. by-products in several industries including nutraceutical and pharmaceutical.
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