Abstract
An antifungal bioemulsifier compound was produced from a novel strain of Bacillus thuringiensis pak2310. To accentuate the production and as the first step to improve the yield, a central composite design (CCD) was used to study the effect of various factors like minimal salts (1X and 3X), glycerol concentration (2% and 4%), beef extract concentration (1% and 3%), and sunflower oil concentration (2% and 4%) on the production of bioemulsifier molecule and to optimize the conditions to increase the production. The E 24 emulsification index was used as the response variable as the increase in surfactant production was seen to be proportional to increased emulsification. A quadratic equation was employed to express the response variable in terms of the independent variables. Statistical tools like student's t-test, F-test, and ANOVA were employed to identify the important factors and to test the adequacy of the model. Under optimum conditions (1X concentration of minimal salts (MS), 2.6% glycerol (v/v), 1% beef extract (w/v), and 2% sunflower oil (v/v)) a 65% increase in yield was produced.
Highlights
The entire dynamics of the world is based on chemical reactions of big and small molecules alike
The bioemulsifier producer Bacillus thuringiensis pak2310, isolated from diesel contaminated soil with NCBI GenBank accession number JF512478, was grown in nutrient broth supplemented with 1% glycerol
Glycerol was found to be the best carbon source among all the three polyols that were taken for the study as it showed significant emulsification kinetics than the other counterparts (Figure 1)
Summary
The entire dynamics of the world is based on chemical reactions of big and small molecules alike. Investigation into the origin of life revealed that certain small molecules are the building blocks of even complex organisms In this world of chemicals, one could figure out that certain harmful molecules can be substituted with ecofriendly biomolecules. The most used group of chemicals that are present in almost all the products around us are the surfactants or the surface active agents. Concentration (CMC), which means that only less amount of bioemulsifier is needed to form micelles when compared to the most used commercial surfactants of today like sodium dodecyl sulphate [6]. The scope of this study is to improve the production of the bioemulsifier from the chosen isolate by optimizing the chemical factors or the media composition of the production medium, employing statistical methods. The significant interactions or relative importance of all interactions can be identified with only less number of experiments [23,24,25]
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