Abstract
Abstract The effect of auxin and cytokinin on shoot tip explant exudation, and the effect of such exudation on the growth of tobacco callus, were studied. Shoot tips of Rubus sp. ‘Dirkson Thornless’ (blackberry), Juglans nigra L., Euphorbia Lathyris L., and Hibiscus Rosa-sinensis L. were cultured on Murashige and Skoog medium. Rhododendron ‘P.J.M. Hybrids’ shoot tips were cultured on Anderson medium. Media contained a factorial combination of IBA and BA (2iP for rhododendron), each at 0, 1, and 10 µM. IBA reduced stem necrosis of hibiscus and rhododendron shoot tips, whereas BA increased length of browning of hibiscus shoot tips on average of 1.1 mm. The shoot tips were removed after 1 week and replaced with tobacco callus (Nicotiana tabacum L. ‘McNair 944’). After 3 weeks, callus growth was reduced on media that previously had black walnut and Euphorbia shoot tips (2939 and 532 mm3 of visible stain). Blackberry, hibiscus, and rhododendron shoot tips had no influence on tobacco callus growth (576, 0, and 1046 mm3 of visible staining). Chemical names used: 1H-indole-3-butyric acid (IBA); N-(phenylmethyl)-1H-purin-6-amine (BA); N-(3-methyl-2-butenyl)-1H-purin-6-amine (2iP); and 1H-indole-3-acetic acid (IAA).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
