Abstract
Carbon nanomaterials (CNMs) have attracted a great deal of research interest for their potential application in plants. Some types of CNMs have exhibited the ability on regulating plant growth of plants, which showed a promising future in agriculture. However, detailed mechanism of their regulation on plant growth is still not well characterized especially at the molecular level. In this study, our objective is to study the regulation mechanism of carbon nanoparticles (CNPs) on tobacco callus growth at protein level. During the incubation period, proteomic profiling of tobacco callus was investigated by using the isobaric tags for relative and absolute quantitation labelling (iTRAQ) coupled with 2D-LC MS/MS. The function of differentially expressed proteins was achieved by using a gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) analysis with InterproScan, and qRT-PCR was used to support the results of the proteomic data. The results showed that the growth of tobacco callus was enhanced by CNPs, with the optimal concentration at 50 mg/L. The expression levels of 198 cellular proteins were significantly up- or down-regulated after CNPs treatment. Some differentially expressed proteins were located in mitochondria and involved in calcium-mediated signaling pathway. The proteomic profile changes provided more deeper understanding of the highly complex regulatory mechanisms in tobacco callus exposed to CNPs.
Highlights
Carbon nanomaterials (CNMs) have attracted a great deal of research interest for their potential application in plants
We demonstrated carbon nanoparticles (CNPs) have the ability to enhance the growth of tobacco callus
The proteomic profile changes of tobacco callus provided a deeper understanding of the highly complex regulatory mechanisms in tobacco callus exposed to CNPs
Summary
Carbon nanomaterials (CNMs) have attracted a great deal of research interest for their potential application in plants. Nanotechnology is the technology for studying the properties and applications of materials in the nanoscale (mainly 1-100 nm) range. With the development of nanotechnology, the application of some CNMs attracted the attention of plant biologists, especially for some beneficial regulation on plant growth [1,2,3]. Some researches has shown the adverse reaction on plants from exposure to a range of CNMs, the effects exerted by the CNMs always involve a high exposure concentration specific to plant species and growth environment [4]. Plenty of studies reported the positive effects of low-dose CNMs on plant growth and development [8]. More studies would be necessary to reveal the mechanism of CNMs on plants
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