Response of soil micro-food web to nutrient limitation along a subtropical forest restoration

Abstract

Forest ecosystem productivity and function is strongly influenced by the interaction between soil organisms and their resource use that can be impeded by an imbalance of ecological stoichiometry. Soil microorganisms are known to have an important role in biogeochemical cycling which is strongly influenced by ecological stoichiometry. However, there is limited understanding of how soil micro-food web respond to stoichiometric imbalances during forest restoration. Here, we investigated the effect of forest restoration on soil physio-chemical properties and the structure and function of soil micro-food web along a chronosequence of transformation stages: (i) early stage monoculture plantation of Chinese fir (Cunninghamia lanceolata) comprised of three age classes (5, 10 and 20 years); (ii) mid-stage conifer-broadleaved mixed forest; and (iii) late-stage mixed species broadleaved forest in south China. Results showed that forest restoration from C. lanceolata monocultures to mixed species broadleaved forest significantly increased soil organic carbon and total nitrogen. Soil bacteria, fungi, protists and nematodes abundance increased and the co-occurrence networks of soil biota became more complex and stable along the restoration chronosequence. In contrast, soil nitrogen and phosphorus limitations, particularly phosphorus limitation, increased along the chronosequence. In addition, soil exoenzyme activity suggested that the microbial investment in resource acquisition shifted from C- to nutrient-acquiring enzymes from the earlier to the later restoration stages. Availability of soil resources (e.g., dissolved organic carbon, ammonium, and available phosphate) appeared to have an important role in regulating soil food web composition, structure and stability during forest restoration. We conclude that nutrient limitation, particularly phosphorus limitation, likely has an important role in determining the stability of soil food webs during forest restoration. These findings contribute to our understanding of the relationships between soil nutrient limitation and soil micro-food web, and have implications for carbon sequestration through forest restoration and management in southern China.