Response of soil bacterial community to bioaugmentation with a plant residue-immobilized bacterial consortium for crude oil removal

Abstract

Both the crude oil removal efficiency of the Eichhornia crassipes dried straw-immobilized bacterial consortium and shifts in soil bacterial community in response to the bioaugmentation strategy were unmasked in this study. After 30 days of bioremediation, total petroleum hydrocarbon in soil was determined and immobilized consortium showed a removal percentage (51.7%) better than either Eichhornia crassipes dried powder (37.0%) or bacteria solution (36.0%) alone. Bacterial community and soil properties analyses demonstrated that the relative abundances of Cytophagales and Rhizobiales increased with increasing total organic carbon and total nitrogen contents because of the addition of Eichhornia crassipes dried powder. The genus Burkholderia which may play a key role in hydrocarbon degradation among the inoculated bacterial consortium proliferated when immobilized on the Eichhornia crassipes dried powder. Such a cell immobilization technology using plant residue materials as carriers helps to improve soil fertility and mitigate competition between indigenous and inoculated microorganisms for nutrients, which offers a promising way to enhance the removal of crude oil from contaminated soils.