Abstract
Rat adrenal quarters were incubated in a stream of medium (superfusion) and the content of corticosterone in the outflow (superfusate) was continuously recorded by an automated fluorometric method. The apparatus was standardized with known amounts of corticosterone; a linear relationship was established between the amount of corticosterone and the area under the peak of fluorescence on the record. The steroidogenic response to a standard dose of ACTH varied from day to day; however, the ratio of responses to 2 doses of ACTH was relatively constant. The total amount of corticosterone formed in response to a given total dose of ACTH was approximately the same in spite of up to a 48-fold change in the rate of administration of the ACTH. The steroidogenic response increased with the dose of ACTH up to the highest dose tested, 200 U/100 mg tissue, without a leveling off of the dose response curve, in contrast to the curve obtained in static in vitro incubations. In confirmation of the results of others, the re...
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