Abstract
AbstractChitin deacetylases (CDAs) are critical enzymes required for chitin degradation, which play an important role in modifying the physical properties of insect cuticles and peritrophic matrices. A novel chitin deacetylase gene (hccda5) was identified from Hyphantria cunea. The recombinant expressed HcCDA5 protein, containing a chitin deacetylase domain (CDAD), did not show chitin deacetylase activity, but instead showed a strong chitin‐binding activity. Transcriptional analysis using qRT‐PCR indicated that hccda5 was mainly expressed in the midgut of H. cunea larvae. Feeding H. cunea larvae with Bt (Bacillus thuringiensis) Cry1A protein induced its downregulation. The gene of hccda5 was successfully silenced by different dsRNAs in insect cell line (Spodoptera frugiperda line, Sf9), which provided a tool to study the function of an expressed gene in insect cell line and laid a foundation for RNAi of hccda5 in H. cunea larvae.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
