Response of aerobic activated sludge to edible oil exposure: Extracellular polymeric substance (EPS) characteristics and microbial community

Abstract

Aerobic activated sludge is widely used to degrade edible oil wastewater in wastewater treatment plants. During this process, the observed poor organics removal performance might be caused by poor sludge settling performance, which might be influenced by extracellular polymeric substances (EPS) and the structure of the microbial community. However, this hypothesis was not confirmed. Thus, this study investigated the response of activated sludge to 50% and 100% edible oil exposure in comparison to glucose, focusing on organics removal performance, characteristics of sludge, EPS, and microbial community structure. Results showed that both concentrations of edible oil influenced the systems’ performance, although 100% edible oil showed more significant negative effects than 50% edible oil. The mechanisms behind the influence of edible oil on the aerobic activated sludge system and the differences between the different concentrations of edible oil were revealed. The worse system performance in the edible oil exposure system was due to the worse sludge settling performance, which was significantly affected by edible oil (p < 0.05). The sludge settling performance was mainly inhibited by promoting the formation of floating particles and the enrichment of filamentous bacteria in the 50% edible oil exposure system; biosurfactant secretion was also speculated as the reason, in addition to the above factors, in the 100% edible oil exposure system. The macroscopic largest floating particles, highest total relative abundance of foaming bacteria and biosurfactant production genera (34.32%), lowest surface tension (43.7 mN/m), and highest emulsifying activity (E24 = 25%) of EPS in 100% edible oil exposure systems provide strong evidence.