Respiratory quotient and ammonia excretion in Tilapia mossambica

Abstract

Tilapia mossambica (Peters), acclimated to and tested in fresh water at 30°C, maintained a routine respiratory quotient (R Q) of about unity and an ammonia quotient (A Q) (Vol. NH2/Vol. O2) of about 0.2 at high ambient oxygen concentrations. At low oxygen concentrations (below 2 ppm) R Q and A Q increased sharply to values of 8 and 1, respectively (at 0.6 ppm), indicating a close relationship of increase in anaerobic energy utilization and increase in protein metabolism at inadequate oxygen concentrations. T. mossambica (8 cm), exercised continuously with intervening sampling and flushing stops for 6 h, at a swimming speed of about 2 body lengths/sec, derived some anaerobic energy throughout the exercise (R Q: 1.2), utilizing more protein the longer the exercise. The coupling of the increased protein metabolism and anaerobic energy utilization may be of advantage in preventing acidosis and also in conserving sodium (Na+) in fish.