Abstract
Respiratory burst oxidase homologue‐dependent H2O2 (RBOH-H2O2) has been reported to act as a signal during heat stress (HS) memory in heat resistant tomato (HRT). However, function of RBOH-H2O2 in heat sensitive tomato (HST) and the difference between HRT and HST for RBOH-H2O2 during HS memory remain unclear. In this study, RBOH-H2O2 not only acts as a signal enhancing the expression of HS response genes during HS memory but also regulating antioxidant enzymes’ activity controlling the total H2O2 at a level conducive to HS memory, which contributes to tomato seedlings maintaining a lower level of total H2O2 during the future HS challenge and enhances the maintenance of acquired thermotolerance (MT) in HST. Compared with HRT, the HST needs longer duration of HS-acclimation to obtain HS memory. Moreover, higher concentration of diphenylene iodonium (DPI, an NADPH oxidase inhibitor) was needed in HST during recovery after extended HS-acclimation, which increases the MT of HST. Additionally, although extended HS-acclimation results in the loss of MT, proper inhibition of RBOH-H2O2 after extended HS-acclimation can alleviate the oxidative stress. However, stronger inhibition of RBOH-H2O2 will result in the decrease of MT. In conclusion, RBOH-H2O2 acts as signal during HS memory in HST and this signal for HST shows slower in response to HS-acclimation and DPI than HRT.
Published Version
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