Resonance Rayleigh scattering spectra of interaction of sodium carboxymethylcellulose with cationic acridine dyes and their analytical applications

Abstract

In the near neutral media, sodium carboxymethylcellulose (NaCMC) can react with a cationic acridine dyes such as acridine yellow (AY) and acridine orange (AO) to form an ion-association complex. As results of the reaction, intensity of resonance Rayleigh scattering (RRS) is enhanced greatly and new RRS spectra appeared. The maximum scattering peaks exist at 285 nm for AY–CMC and 315 nm for AO–CMC. There is linear relationship between the relative intensity of RRS (Δ I) and concentration of NaCMC in the range of 0–1.5 μg ml −1 for AY system and 0–3.0 μg ml −1 for AO system. The method for determination of NaCMC has high sensitivity. The detection limits (3 σ) are 6.0 ng ml −1 for AY and 41.7 ng ml −1 for AO. Moreover, effects of foreign substances on determination of NaCMC have been investigated. It shows that the methods have good selectivity. A new method for the determination of NaCMC with AY or AO by RRS technique has been proposed. The method is sensitive, simple and fast.