Abstract
The throughput and label-free multianalyte interrogation ability provided by surface plasmon resonance imaging (SPRi) sensors has gained considerable interest in biochemical analysis. Here, we report an optical platform based on polarization contrast modulation, in which a three-dimensional plasmonic microwell array is used as a sensing chip to eliminate background resonance and greatly enhance the electromagnetic field response in the microwell structure by confining the SPR. In addition, the sensor can provide an extremely high contrast and signal-to-noise ratio and can achieve a very high sensitivity response (1.52 × 105 pixel / RIU). Additionally, the signal-to-background ratio is improved to 40. The low-concentration limit of detection can reach 5.38 × 10 − 7 RIU, which is 1 order of magnitude improvement. In biomolecular interaction analysis, the sensor can detect a 5.12 × 104-fold diluted bovine serum albumin antibody solution. The special chip architecture and high image quality introduce an array screening method using SPRi detection.
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