Abstract
Periodic nanostructures fabricated by Nanoimprint Litography (NIL) in low-cost plastic substrates and coated with thin gold film were explored for enhanced surface plasmon resonance imaging (SPRi) detection. Rigorous coupled-wave analysis was used to model the SPRi response of these nanostructured surfaces. Two-dimensional nanogratings and nanogrooves were fabricated on Zeonor 1060R(TM) by NIL and followed by metal deposition. The detection of refractive index changes in the dielectric layer due to bulk medium change, DNA immobilization and DNA hybridization events were monitored using SPRi to assess the corresponding signal amplification. The results indicate target-dependent sensitivity enhancement which is maximized for the detection of biomolecular binding events. The 500 nm period nanogrooves provided a 4 times SPR signal amplification compared to the conventional uniform gold film on SF-11 glass for DNA hybridization detection. Our work demonstrates that the use of nanoimprinted plastic substrates provides a low-cost solution for the SPR-based detection with sensitivity that meets the requirements in practical diagnostic applications.
Highlights
The use of surface plasmon resonance imaging (SPRi) has emerged in the last few years as a promising alternative to traditional fluorescence-based assays for parallel, real-time, labelfree detection of binding interactions in several fields
Periodic nanostructures fabricated by Nanoimprint Litography (NIL) in low-cost plastic substrates and coated with thin gold film were explored for enhanced surface plasmon resonance imaging (SPRi) detection
Two-dimensional nanogratings and nanogrooves were fabricated on Zeonor 1060RTM by NIL and followed by metal deposition
Summary
The use of surface plasmon resonance imaging (SPRi) has emerged in the last few years as a promising alternative to traditional fluorescence-based assays for parallel, real-time, labelfree detection of binding interactions in several fields. This is evidenced by the growing number of publications in the fields of medical diagnostics, environmental monitoring, and food safety and security. Periodic metal nanogratings and nanogrooves deposited on nanostructured template fabricated on low-cost plastic substrates are investigated both numerically and experimentally for enhanced SPRi detection of various targets ((i) bulk medium refractive index change due to different salt concentrations in water, (ii) monolayer formation due to DNA immobilization on gold surface, and (iii) biomolecular interactions due to DNA hybridization event)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
