Abstract
Two chemometrics-assisted UV spectrophotometric methods were proposed for the resolution of ternary mixtures without any chemical pretreatment. The first method is based on modification of H-point standard addition method which permits simultaneous analysis of three species from a unique calibration set by making the simultaneous addition of the three analytes. Quotient between the spectra of aspirin, atorvastatin, and clopidogrel was obtained and the results showed that simultaneous determination of aspirin, atorvastatin, and clopidogrel can be obeyed in the linear range 2.5–20 μg mL−1 of aspirin, 2.5–17.5 μg mL−1 of atorvastatin, and 2.5–20 μg mL−1 of clopidogrel in ternary mixture. The second method is based on the combination of the first derivative spectra and Cramer's matrix rule. In the matrix calculation, clopidogrel has zero crossing point at 316.8 and 212 nm, while for atorvastatin the zero crossing point at 250 nm where the matrix is greatly simplified and easily solved. The linear concentration ranges were 2.5–20 μg mL−1 aspirin, 2.5–17.5 μg mL−1 atorvastatin and 2.5–20 μg mL−1 clopidogrel in ternary mixtures. The results proved that the simultaneous determination of aspirin, atorvastatin, and clopidogrel could be obeyed. Both methods were applied for capsules containing the three ingredients and results were in good concordance with alternative liquid chromatography.
Highlights
Aspirin (ASP, acetylsalicylic acid) is used as an analgesic, antipyretic, anti-inflammatory medication and an antiplatelet agent
Atorvastatin calcium (ATOR), [R-(R∗,R∗)]-2-(4-fluorophenyl)-β,δ,dihydroxy5-(1-methylethyl)-3-phenyl-4 [(phenylamino)carbonyl]lH-pyrrole-1-heptanoic acid, calcium salt (2 : 1) trihydrate is an inhibitor of 3 hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase
Chemometric methods essentially reduce the solvents used and the duration of analysis by doing most of the works on the front desk using microcomputers with appropriate softwares on the primary data generated in the laboratory work
Summary
Aspirin (ASP, acetylsalicylic acid) is used as an analgesic, antipyretic, anti-inflammatory medication and an antiplatelet agent. Hajian and Afshari [14] developed a new method for the simultaneous analysis of a ternary mixture by combination of double divisor-ratio spectra derivative and Hpoint standard addition method (HPSAM). It is a modification of the standard addition method and has been frequently applied in spectrophotometry [17,18,19,20,21,22,23] It was developed for resolution of binary mixture with simultaneous addition of both analytes [24]. The HPSAM with simultaneous standard addition of three analytes and combination of first derivatives spectra and Cramer’s matrix rule has been applied for the simultaneous analysis of ASP, ATOR, and CLOP in their ternary mixture. HPLC method was used for simultaneous determination of these compounds in the same ternary mixture and pharmaceutical formulation [7]
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