Abstract
Growing resistant cultivars is the best method of protecting the crops against Potato virus Y (PVY). There are a few sources of PVY resistance/tolerance in tobacco acquired through mass selection, X-ray induced mutagenesis and introgressions from wild Nicotiana species. Here, we compare major sources of PVY resistance/tolerance in inoculation tests using ten PVY isolates collected in Central Europe (Poland and Germany) and differing with their virulence. The diversity of collected isolates was confirmed by DAS-ELISA tests and two PCR assays targeting the most common recombination sites in the PVY genome. We used these isolates in inoculation tests on five resistant cultivars ‘V.SCR’, ‘PBD6’, ‘TN86’, ‘VAM’, ‘Wiślica’, a tolerant breeding line ‘BPA’ and four susceptible cultivars ‘BP-210’, ‘K326’, ‘NC95’, ‘Samsun H’. None of the tested cultivars/breeding lines showed universal resistance against all ten isolates. However, ‘VAM’ and ‘Wiślica’ appeared to be the most effective sources, as they showed no symptoms and gave negative DAS-ELISA tests for four out of ten tested PVY isolates. In contrast, tolerance of the breeding line ‘BPA’ was effective against all tested isolates, because inoculation did not lead to development of full disease symptoms in that breeding line.
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