Abstract
Potato virus Y (PVY) is a highly pathogenic virus, causing enormous economic losses in potato (S. tuberosum) crop. Three isolates of PVY were obtained from naturally infected potato plants showing mosaic; yellowing and vein necrosis symptoms, during 2017-2018 growing seasons at certain locations of El-Beheira and Alexandria governorates, Egypt. PVY could be easily transmitted mechanically by aphids. Detection of the PVY-3 in different organs of infected Nicotiana glutinosa plants by Indirect-ELISA; Dot blot immunoassay (DBIA) and Tissue blot immunoassay (TBIA), indicated the possibility of using these methods for viral detection. Egyptian PVY (MK376452) isolate was of close homology to PVY isolated from South Africa. The other Egyptian isolates were found to be close to a French PVY (KJ741115) isolate. There were variations on comparing nucleotide and amino acid sequences; however, nucleotide sequencing could be more reliable. Application of sequence inspection allowed us to identify the PVY isolates by phylogenetic analysis. Phylogenetic analysis of the genomic coat protein (CP) from 24 PVY isolates registered in GenBank indicated the presence of relationships between each other's. This reflected the high degree of genetic variability among our local Egyptian isolates. The aims of the current work were to; isolate and detect PVY from naturally infected potato plants in northern Egypt, characterize the PVY isolates using different assays, detect PVY in different organs of infected potato plants, study the CP gene of the PVY isolates using Reverse transcription-Polymerase Chain Reaction (RT-PCR), and register these isolates in GenBank.
Highlights
Potato virus Y (PVY) is one of the most destructive plant pathogens all over the world
Three PVY isolates were recovered from leaf samples of infected potato plants exhibiting mosaic and vein necrosis symptoms
Two isolates of PVY were isolated from El-Behaira governorate (Abo Homos reigion (PVY-1) and El Nobaria regions (PVY-2); whereas, one isolate was isolated from the farm of Faculty of Agriculture-Saba Basha, Abis, Alexandria governorate (PVY-3)
Summary
PVY is one of the most destructive plant pathogens all over the world It caused a lot of damage in many economically important crops such as; potato, tomato, tobacco and pepper. PVY represented one of the highest threats to potato production worldwide, and it reduced crop yields up to 90% (Ivanov et al, 2014). Likewise, it has long been recognized as a threat to potato cultivation in Egypt (El-Borollosy, 2015; Abdalla et al, 2018). PVY diagnosis was based on serological testing such as indirect ELISA, which used specific polyclonal antiserum to this virus (Hamza et al, 2018). The objectives of the current study were to detect and isolate PVY from infected potato plants in Alexandria and ElBeheira governorates, to characterize these PVY isolates using host range; symptomology, mode of transmission, and serological studies including; Indirect ELISA, TBIA, DBIA, to detect PVY in different organs and at different periods of infection of potato plants, to study these PVY isolates using a molecular techniques as RT-PCR, and to register these isolates in the GenBank
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