Resistance to controlled thermal stress and tolerance to sperm cryopreservation of two groups of boars

B Stankovic,J Bojkovski,B Petrujkic,N Delic,S Hristov,N Maksimovic

doi:10.2298/bah1201059s

B Stankovic, J Bojkovski + Show 4 more

Open Access

https://doi.org/10.2298/bah1201059s

Copy DOI

Abstract

The aim of this study was to find out is it possible to presume success of boars sperm cryopreservation, based on controlled exposure to heat stress and to investigate in vitro quality parameters of frozen/thawed sperm of previously selected 6 boars with good results (group 1) and 6 boars (group 2) with bad results on thermo resistance test (TT). In this investigation, ejaculates of 12 chosen of 75 AI boars (Landrace, Large White, Pietrain and Durock breeds) from SVC Velika Plana (Serbia) were used. Tolerance to heat stress was performed by Schaetz (1963) method. Ejaculates were extended with Androhep plus (1:1) and kept during 60 minutes on 41oC. In cryopreservation of boars semen Westendorf et al. (1975) method, modified by Bwanga et al. (1990). Very significant changes of progressive motility rate were observed after spermatozoa exposure to controlled thermal stress. High correlation coefficient of progressive motility rate of both groups of boars, but higher in group 2 for progressive motility rate after thawing and after heat stress treatment was established. Average progressive motility rate of all 12 boars did not differ before and after thawing. Presence of boars of all breeds represented in the SVC (Large White, Landrace, Duroc and Pietrain) in both quality groups confirms the assumption that suitability for cryopreservation of sperm is individual trait. According data analysis, sperm deep freezing success requires previous selection for potential donors, which have to be consider standard quality parameters testing and controlled stress exposure tests.

Highlights

IntroductionThe use of frozen/thawed boar semen makes less than 1% of all artificial insemination (AI) in swine population of the world (Wagner and Thibier, 2000)
The use of frozen/thawed boar semen makes less than 1% of all artificial insemination (AI) in swine population of the world (Wagner and Thibier, 2000).Disadvantages and limitations of frozen/thawed boar semen AI are primarily related to low fertilizing rate (40-70%), and litter size (7-10 piglets), comparing to diluted semen inseminations (Johnson, 1985; Almlid and Hofmo, 1996)
Considering available data, it makes sense to presume that ability to preserve intact cell membrane of the boar spermatozoa in extreme low temperature shock conditions and its elasticity to bear all types of stress might be very useful in sperm cryopreservation success prediction

Summary

Introduction

The use of frozen/thawed boar semen makes less than 1% of all artificial insemination (AI) in swine population of the world (Wagner and Thibier, 2000). Disadvantages and limitations of frozen/thawed boar semen AI are primarily related to low fertilizing rate (40-70%), and litter size (7-10 piglets), comparing to diluted semen inseminations (Johnson, 1985; Almlid and Hofmo, 1996). Considering available data, it makes sense to presume that ability to preserve intact cell membrane of the boar spermatozoa in extreme low temperature shock conditions and its elasticity to bear all types of stress might be very useful in sperm cryopreservation success prediction. The aim of this study was to find out is it possible to presume success of boars sperm cryopreservation, based on controlled exposure to heat stress and to investigate in vitro quality parameters of frozen/thawed sperm of previously selected 6 boars with good results (group 1) and 6 boars (group 2) with bad results on thermo resistance test (TT)

Objectives

Methods

Results

Conclusion