Abstract
Objective To study the resistance to acute reaction in composite tissue allotransplan-tation by shRNA interference from CD40 costimulatory molecule in rat lymphocytes. Methods The in-bred SD rats were chosen as donors and inbred Wistar rats as recipients. Twenty-seven recipients were divided into 3 groups randomly after allogeneic leg transplantation. On the day after transplantation, rats in group A were injected with 600 μL of Sofast-siCD40-2/pSilencer, rats in group B received 600 μL of Sofast-pSilencer 4.1-CMV neo, and rats in group C received injection of 600 μL of sodium chloride. The survival and rejection to grafts were observed. The rats which survived 7 days after operation were given the test of mixed lymphocyte reaction (MIR). Results The mean survival time of limb allografts in group A was obviously longer than that in the other two groups( P ＜ 0.01 ) . Rejection to grafts was not observed in group A, but rejection e-merged shortly after transplantation in the other two groups. Rats in group A had a low reaction to the donors' lymphocytes. Conclusion Without any immnosuppressants, shRNA interference from CD40 costimula-tory molecule can resist acute rejection in limb allotransplantation in rats. Key words: Tissue; Transplantation; RNA interference; Graft survival; Graft rejection
Published Version
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