Abstract
The aim of the present study was to determine the susceptibility status of Culex pipiens pipiens populations against deltamehtrin insecticide. Larvae of Culex pipiens pipiens were collected from three breeding places in Northern and Southern Tunisia between 2003 and 2005. Early third and late fourth instars were tested against deltamethrin pyrethroid insecticide. Cross-resistance with DDT resistance was evaluated in studied samples to estimate the role of target site insensitivity and two synergists including piperonyl butoxide (Pb) and S,S,S-tributyl phosphorotrithioate (DEF) were used to estimate the role of detoxification enzymes. Our results revealed that the level of deltamehtrin resistance ranged from 0.67 to 31.4. We also showed the non-involvement of kdr resistance in pyrethroid resistance and no cross-resistance with DDT resistance was detected in all studied populations including the most resistant one. Synergists study on the resistant population (sample # 1) showed the involvement of CYP450 in the recorded resistance to the deltamethrin insecticide. The results obtained from this study should be considered in the current control programs to combat mosquitoes in Tunisia.
Highlights
Culex mosquitoes are known as main vectors of lymphatic filariasis and several viral pathogens[1] including West Nile encephalitis which regularly strikes Tunisia, North Africa[2]
Cross-resistance with DDT resistance was evaluated in studied samples to estimate the role of target site insensitivity and two synergists including piperonyl butoxide (Pb) and S,S,S-tributyl phosphorotrithioate (DEF) were used to estimate the role of detoxification enzymes
In the present study, three field-populations of Culex pipiens pipiens were collected from different parts of Tunisia
Summary
Culex mosquitoes are known as main vectors of lymphatic filariasis and several viral pathogens[1] including West Nile encephalitis which regularly strikes Tunisia, North Africa[2]. African Health Sciences Vol 18 Issue 4, December, 2018 enzymes are well documented[7] and have associated with pyrethroid resistance in mosquitoes[8,9,10]: cytochrome P450 monooxygenases (CYP450), carboxyl/choline esterases (CCEs) and glutathione-S-transferases (GSTs). Moderate and high level of resistance to pyrethroids in Culex mosquitoes from Tunisia[4,13]. Cross-resistance with DDT resistance was evaluated in studied samples to estimate the role of target site insensitivity and two synergists including piperonyl butoxide (Pb) and S,S,S-tributyl phosphorotrithioate (DEF) were used to estimate the role of detoxification enzymes
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