Resistance of transgenic zoysiagrass overexpressing the zoysiagrass class II chitinase gene Zjchi2 against Rhizoctonia solani AG2-2 (IV)

Abstract

Zoysiagrass (Zoysia japonica Steud.) is an important turfgrass species used in golf courses and athletic fields. However, zoysiagrass is susceptible to large patch disease caused by Rhizoctonia solani AG2-2 (IV). Chitinases are pathogen-related (PR) proteins induced by viruses, bacteria, and fungi that hydrolyze chitin. Recently, we isolated a class II chitinase gene (Zjchi2) from zoysiagrass. The purified recombinant Zjchi2 showed broad-spectrum activity against various fungi, including R. solani AG2-2 (IV). In the current study, we generated transgenic zoysiagrass overexpressing Zjchi2 and then verified the resistance of transgenic plants to R. solani AG2-2 (IV). Polymerase chain reaction and Southern blot hybridization showed the integration of transgenes in zoysiagrass genomes and constitutive expression of Zjchi2, respectively. Antifungal activity was enhanced significantly in the transgenic zoysiagrass compared with wild-type plants. To our knowledge, this report is the first on the antifungal activity of a class II chitinase in transgenic zoysiagrass.