Resistance and virulence determinants of faecal Salmonella spp. isolated from slaughter animals in Benin

Esther Deguenon,Evelyne Lozes,Nana Maman,Fidélia Djegui,Jerrold Agbankpe,Roula M Abdel-Massih,Jacques Dougnon,Victorien Dougnon,Lamine Baba-Moussa

doi:10.1186/s13104-019-4341-x

Esther Deguenon, Evelyne Lozes + Show 7 more

Open Access

https://doi.org/10.1186/s13104-019-4341-x

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Abstract

ObjectiveSalmonella spp. are one of the leading foodborne pathogens worldwide naturally found in the intestines of many animals. People that are in direct contact with the infected animals or their cages may become ill. The aim of this study was to determine the prevalence, antibiogram and virulence genes associated with Salmonella serovars from fecal samples of animals intended for consumption in Southern Benin.ResultsOut of a total of 406 samples, 2.46% were positive. The isolates identified were multidrug-resistant Salmonella spp. to penicillins, first generation cephalosporins and some aminoglycosides. All Salmonella isolates produced invA gene of 284 bp, fimA of 85 bp and stn of 260 bp. The spvC gene (571 bp) was present in 10% of the isolates whereas the spvR gene (310 bp) was found in 20% of the isolates. The control strain possessed all the tested genes. The invA gene implies that strains are able to invade epithelial cells. The fimA and stn genes present in all isolates show that they are capable of causing gastrointestinal illness in humans. The presence of spvC and spvR genes suggests the possibility of these strains to produce toxins.

Highlights

Salmonella is a genus of rod-shaped Gram-negative bacteria of Enterobacteriaceae family
The importance of Salmonella spp. as potentially dangerous bacteria can be influenced by both multidrug resistance and presence of virulence genes
Salmonella enterica has several pathogenicity islands in its genome, which are genetic elements that harbor genes associated with virulence [20]

Summary

Results

Sample collection A total of 406 samples of slaughter animal faeces were collected. Additional file 1: Figure S2 shows the appearance of a faecal sample inoculated on XLD agar. The prevalence of Salmo- P17 nella isolated from faeces of slaughter animals was 2.46% P19 during the period from May to September 2018. Molecular identification All Salmonella isolates were positive for the presence of invA genes (284 bp), fimA (85 bp) and stn (260 bp). The reference strain was positive for all genes and served as control. Salmonella strain P19 found in faeces of local hens sold on the market was the only with all the virulences genes.

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