Abstract
Aim:The aim was to characterize Salmonella enterica serovar Gallinarum isolated from backyard poultry by polymerase chain reaction (PCR) detection of virulence genes invasion (invA) and Salmonella plasmid virulence C (spvC).Materials and Methods:Two strains of Salmonella serovar Gallinarum isolates used in this study were obtained from an outbreak of fowl typhoid in backyard Vanaraja fowl. PCR technique was used for detection of invA and spvC genes using standard methodology. The invA PCR product from one representative isolate was sequenced and compared with other related Salmonella serovars in GenBank data.Results:Salmonella Gallinarum produced expected amplicons of invA and spvC gene products. Nucleotide sequence of 285 bp invA gene was deposited in GenBank with accession no. KX788214. Sequence analysis of invA gene was found conserved in Salmonella serovars and demonstrated 100% homology with closely related serovars of Salmonella.Conclusion:Invasion gene (invA) was found to be highly conserved in Salmonella Gallinarum and highly similar with closely related serovars. The isolates also contained plasmid-mediated spvC gene indicating possession of virulence plasmid.
Highlights
Fowl typhoid (FT) is disease of major economic significance in many countries of Asia, Africa, Central and South America [1]
Sequence analysis of Invasion gene (invA) gene was found conserved in Salmonella serovars and demonstrated 100% homology with closely related serovars of Salmonella
Amplicons of invA and Salmonella plasmid virulence C (spvC) virulence genes were observed in agarose gel as ~284 bp and ~571 bp products, respectively (Figure-1)
Summary
Fowl typhoid (FT) is disease of major economic significance in many countries of Asia, Africa, Central and South America [1]. It is an endemic disease of poultry in India with occasional outbreaks [2,3,4]. The majority of virulence genes of Salmonella are clustered in a region distributed over the chromosome, called Salmonella pathogenicity islands (SPI). One large plasmid of approximately 85 kb in Salmonella Gallinarum have the ability of strains to produce high mortality in chickens [6] and Salmonella plasmid virulence (spv) locus that carries the spv genes were reported to be present
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
