Residual Estimation of Isoproturon, Atrazine and Grain Protectants in Stored Wheat Grains

Abstract

This study was carried out to see the occurrence and level of pesticide residues in stored wheat grain samples. Wheat grains were collected from different godownslocated in Faisalabad district. Weedicide residues in wheat grains were analyzed b y using thin plate liquid chromatography and validated high performance liquid chromatography . On the basis of extent of inhibition of the Hill reaction, it was found that the level of atrazine was (0-0.028 ngkg -1 ) and the level of isopproturon was (0 -0.024 ngkg -1 ) in test samples. The calculated values were below the Maximum Residue Limit (MEL) i.e.100 ngkg -1 for atrazine and 2200 ngkg -1 for isoproturon. It was found that presence of herbicide residues in stored wheat was negligible. Further, on wheat grain samples placed in jars and treated with deltamethrin and cypermethrin @0.4 and 0.8% concentration. The persistency data was calculated after 1 hour, 7, 15 and 30 days of treatment. The grain protectants were continuously degraded with the passage of time and after the 30 days the amount of residues of the grain protectant s was found to be very small. It was concluded that the use of the grain protectants in the powder form incombination with other botanicals on stored wheatsamples is feasible to reduce the insect pest in stored grains.